Senior Scientist My client is seeking a Senior Scientist to join their team and lend expertise in cell-free protein expression in bacterial systems. My client offers the independence, resources, and collaborative environment needed to drive innovative research. The ideal candidate will take on the following tasks: Design, execute, optimize, and troubleshoot bacterial cell-free protein expression for a diverse range of target proteins. Optimize extract preparation methods to maximize protein yield and activity. Develop and apply high-throughput screening approaches to evaluate expression constructs, chaperone combinations, and reaction conditions. Characterize expressed proteins for yield, purity, solubility, folding, and functional activity using SDS-PAGE, capillary electrophoresis, ELISA, and related analytical techniques. Maintain detailed lab notebooks and contribute to internal reports, SOPs, and patent disclosures. Stay current with advances in cell-free protein synthesis literature and evaluate new methods, vendors, and platforms. The ideal candidate will possess the following qualities: PhD in Biochemistry, Molecular Biology, Protein Science, or related. Demonstrated hands-on expertise in bacterial cell-free protein synthesis including optimization and troubleshooting. Strong working knowledge of E. coli molecular biology, protein expression, and extract preparation. Proficiency in standard protein analytical techniques (SDS-PAGE, Western blot, ELISA, capillary electrophoresis).Experience with high-throughput and automated screening platforms. Proven ability to lead scientific projects and collaborate across multidisciplinary teams. My client offers a base salary of $115k-135k plus bonus and relocation.
