Scientist II, in vivo Gene Insertion

Company Overview: Founded over a decade ago, CRISPR Therapeutics is a leading biopharmaceutical company focused on developing transformative gene-based medicines for serious human diseases. The Company has evolved from a pioneering research-stage organization into an industry leader, marking a historic milestone with the approval of CASGEVY (exagamglogene autotemcel [exa-cel]), the world's first CRISPR-based therapy, approved for eligible patients with sickle cell disease and transfusion-dependent beta thalassemia. CRISPR Therapeutics is advancing a broad and diversified pipeline across hemoglobinopathies, cardiovascular, autoimmune, oncology, regenerative medicine and rare diseases. The Company continues to expand its leadership in gene editing through the development of SyNTase editing, a novel and proprietary gene-editing platform designed to enable precise, efficient, and scalable gene correction. To accelerate and expand its impact, CRISPR Therapeutics has established strategic collaborations with leading biopharmaceutical partners, including Vertex Pharmaceuticals. CRISPR Therapeutics AG is headquartered in Zug, Switzerland, with its wholly-owned U.S. subsidiary, CRISPR Therapeutics, Inc., and R&D operations based in Boston, Massachusetts and San Francisco, California.

Position Summary: We are seeking highly motivated, talented, and independent researchers to grow our diverse, innovation-focused CRISPR-X team that pioneers novel in-vivo gene editing therapies for human disease. As part of CRISPR Therapeutics, a leading gene editing company, the candidate will be focused on working with our team on developing and enabling in vivo gene insertion across multiple tissue and cell types, with the goal of advancing cutting-edge in-vivo gene editing modalities across a range of different disease indications. The successful candidate will be comfortable working with different gene editing systems, possess a strong immunology and/or synthetic immunology background, and is comfortable moving into new areas of biology as needed. The position requires enthusiasm, adaptability, attention to detail, and a desire to create new medicines for patients.

Responsibilities: Design and execute workflows of conceive, build, and assess in primary cells Requires extensive hands-on knowledge of molecular biology strategies, including advanced cloning methods, experience in handling various primary cell types (including T cells), familiarity with delivery strategies from transfection to viral transduction to novel targeted LNP (tLNP) approaches. Strong sense of teamwork. Collaborate with various groups within CRISPR Tx and outside vendors to advance projects from both an editing and delivery perspective, with the ultimate goal of clinical readiness. Extensive experience in quantitative biology. Iterate on assessing editing efficiency/gene insertion, impact on cell phenotype/construct expression, and ideation of new strategies. Design and execute ddPCR assays to quantify transgene integration efficiency Design and execute T cell phenotyping assays such as multicolor flow cytometry panels Leverage immunology and/or synthetic immunology background to drive innovative editing, delivery, and expression strategies Work cross-functionally to assess editing outcomes in animal models Analyze and present experimental data to a wide range of audiences Generate, manage, evaluate, and maintain critical data in a highly organized manner, providing statistical analysis and troubleshooting where appropriate.

Minimum Qualifications: Extensive prior experience with gene editing and synthetic immunology in an academic and/or industry setting, with a track record demonstrating significant contributions. Depending on level, degrees in Biology, Immunology, Genetics, Bioengineering, or related disciplines (level commensurate with experience and achievement):Scientist II - PhD with minimum of 2-5+ years relevant experience; non-PhD with 10-12+ years progressive, relevant experience Excellent technical skills for cloning, flow cytometry, primary T cell culturing, editing assays, and assessment of T cell phenotype and function Experience managing multiple projects and priorities; ability to jump in to help other projects as needed Strong communicator with a demonstrated record of being a highly collaborative team player within and across multiple departments

Preferred Qualifications: Knowledge and experience with in vivo delivery modalities such as tLNP, AAV, VLP, and others Knowledge and experience with developing novel editing modalities

Location: San Francisco, CA

Type: Full time