Research Technologist- iPSC Cell Culture and Genome Engineering

Position Summary: We are seeking a highly motivated Research Technologist to join a cardiovascular research laboratory focused on human induced pluripotent stem cell (iPSC) models of cardiac diseases inherited. The successful candidate will play a key role in establishing and maintaining iPSC cultures. This position offers an excellent opportunity to gain hands-on experience with cutting-edge stem cell technologies, genome engineering, and translational cardiovascular research in a collaborative academic environment.

Primary Responsibilities: Perform routine maintenance, expansion, cryopreservation, and quality control of human iPSC lines. Differentiate iPSCs into cardiomyocytes and other relevant cell types following established protocols. Isolate and expand single-cell clones and perform molecular validation using PCR, Sanger sequencing, and related techniques. Conduct molecular and cellular assays including DNA/RNA extraction, qPCR, Western blotting, immunofluorescence staining, and flow cytometry as needed. Maintain accurate laboratory records, experimental documentation, and electronic databases. Prepare reagents, maintain cell culture supplies, and ensure compliance with laboratory safety procedures. Assist in data analysis, figure preparation, and presentation of research findings. Participate in laboratory meetings and contribute to collaborative research projects.

Required Qualifications: Experience with mammalian cell culture.

Preferred Qualifications: Bachelor's or master's degree in Cell Biology, Molecular Biology, Genetics, Biomedical Sciences, Biotechnology, or a related field. Experience culturing human iPSCs or embryonic stem cells. Experience with stem cell differentiation, particularly cardiac differentiation. Familiarity with PCR, cloning, DNA sequencing, and molecular biology techniques. Experience with fluorescence microscopy, flow cytometry, or next-generation sequencing is desirable. Prior experience in cardiovascular, stem cell, or genetic disease research. Desired Personal Attributes Strong motivation to learn new techniques. Excellent problem-solving and troubleshooting skills. Ability to manage multiple projects simultaneously. Commitment to maintaining rigorous scientific standards and reproducible research. Interest in translational medicine and cardiovascular genetics. Research Environment Our laboratory investigates the molecular mechanisms underlying inherited cardiac diseases using patient-derived iPSC models, CRISPR-based genome engineering, advanced molecular biology, and functional genomic approaches. The successful candidate will work closely with trainees and collaborators while receiving mentorship in cutting-edge stem cell and genome editing technologies. This position provides outstanding preparation for graduate school, medical school, physician-scientist training, or careers in biotechnology and the pharmaceutical industry. MINIMUM EDUCATION, WORK EXPERIENCE & REQUIRED CERTIFICATIONS Bachelor's Degree 1+ years of relevant experience; or an equivalent combination of education and experience acceptedRequired Certifications: None BACKGROUND CHECKS/CLEARANCES Employment with the University will require successful completion of background check(s) in accordance with University policies. Penn State does not sponsor or take over sponsorship of a staff employment Visa. Applicants must be authorized to work in the U.S.

SALARY & BENEFITS: The salary range for this position, including all possible grades, is $40,608.00 - $58,836.00. Salary Structure - Information on Penn State's salary structure Penn State provides a competitive benefits package for full-time employees designed to support both personal and professional well-being. In addition to comprehensive medical, dental, and vision coverage, employees enjoy robust retirement plans and substantial paid time off which includes holidays, vacation and sick time. One of the standout benefits is the generous 75% tuition discount, available to employees as well as eligible spouses and children. For more detailed information, please visit our Benefits Page. CAMPUS SECURITY CRIME STATISTICS Pursuant to the Jeanne Clery Disclosure of Campus Security Policy and Campus Crime Statistics Act and the Pennsylvania Act of 1988, Penn State publishes a combined Annual Security and Annual Fire Safety Report (ASR). The ASR includes crime statistics and institutional policies concerning campus security, such as those concerning alcohol and drug use, crime prevention, the reporting of crimes, sexual assault, and other matters. The ASR is available for review here. EEO IS THE LAW Penn State is an equal opportunity employer and is committed to providing employment opportunities to all qualified applicants without regard to race, color, religion, age, sex, sexual orientation, gender identity, national origin, disability or protected veteran status. If you are unable to use our online application process due to an impairment or disability, please contact 814-865-1473. Penn State is committed to and accountable for advancing equity, respect, and belonging. We embrace individual uniqueness, as well as a culture of belonging that supports equity initiatives, leverages the educational and institutional benefits of inclusion in society, and provides opportunities for engagement intended to help all members of the community thrive. We value belonging as a core strength and an essential element of the university's teaching, research, and service mission. Federal Contractors Labor Law Poster PA State Labor Law Poster Penn State Policies Copyright Information Hotlines