Summary:
The St-Pierre lab develops and applies new tools for monitoring and interrogating brain activity with millisecond-timescale temporal resolution. We seek an energetic team member to lead our efforts to characterize and apply new light-responsive proteins in vitro and in vivo. The Research Associate will work on two types of tools: (1) genetically encoded voltage indicators (GEVIs), fluorescent biosensors that report voltage as changes in brightness, and (2) optogenetic silencers, light-gated channels that inhibit neuronal firing. Characterization of these tools requires simultaneous electrophysiological recordings and optical excitation/imaging. This position provides exciting opportunities to work with the latest optical technologies and develop new skills in two-photon voltage Job Duties Culture mammalian cells Prepare brain slices, dissociated neurons, or both Conduct experiments that combine electrophysiology and either optogenetic activation or optical imaging (multi-photon fluorescence microscopy) Maintain reagent and tool supply related to the experiments Maintain an electrophysiological rig, and troubleshooting if needed Analyze data using commercial software or customized data pipelines Teach techniques, procedures, and experimental design to other members of the lab Communicate research results in group meetings, national or international conferences Plan, direct and conduct research experiments Evaluate and analyze data Develop research techniques and perform applications required for specific research projects May conduct literature searches and summarize information in an appropriate format for a particular study Document results of experiments and report to principal investigators May supervise, schedule and coordinate the activities of less experienced research staff Perform other job related duties as assigned
Minimum Qualifications:
Master's degree in Basic Science or a related field. Experience in lieu of degree will not be accepted. Three years of relevant experience typically as a Research Assistant, Research Technician or Postdoctoral Fellow/Associate.
Department Specific Criteria:
Must have experience with performing electrophysiology in dissociated neurons, brain slices or bothMust have experience with dissecting rat cortical, hippocampal neurons, or bothMust have experience maintaining an electrophysiology rig
Preferred Qualifications:- Doctoral degree
- Ability to express transgenes in neurons of brain slices using viruses and/or in utero electroporation
- Experience with imaging neural activity (e.g. voltage or calcium) in brain slices or in vivo, using 1P and/or 2P imaging
Work Authorization Requirement: This position is not eligible for visa sponsorship. Candidates must be legally authorized to work in the United States at the time of application and throughout the duration of employment. Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer. NN; SN
