Aptask Global Workforce (AGW) is seeking a Wet Lab Scientist for an Onsite position with a Global Biotechnology Company located in San Diego, CA. This is a 6-12 month contract opportunity. We are seeking a highly motivated and detail-oriented Contract Wet Lab Scientist to join a Discovery Biology team. This role is central to a high-priority binder screening campaign, responsible for executing the bench-level assays required to identify and validate therapeutic candidates for T-cell-based therapies. The successful candidate will serve as a core operator within the screening funnel, focusing on the transition from binder discovery to functional validation and characterizing the potency, specificity, and safety of candidate binders.
Responsibilities:
Perform non-viral engineering of T cells, delivering genetic material into primary human T cells using advanced electroporation or nucleofection platforms Monitor cell viability and recovery, ensuring high-efficiency expression of synthetic receptors before proceeding to functional testing Execute high-throughput screening assays to assess binder affinity and functionality Maintain and expand primary human T cells and Jurkat cell lines using impeccable aseptic techniques Perform multi-color flow cytometry (FACS) to evaluate surface expression levels and phenotypic markers, using software like FlowJo to analyze and interpret data Run a variety of assays to measure T-cell activation, including cytotoxicity assays (using IncuCyte live-cell imaging or LDH release) and cytokine profiling via ELISA, MSD, or Luminex Track T-cell proliferation using dyes like CFSE Record all experimental procedures meticulously in an Electronic Lab Notebook (ELN) to ensure data integrity Requirements: Bachelor's or Master's degree in Immunology, Molecular Biology, Bioengineering, or a related biological science At least 2 years of hands-on wet lab experience, preferably within an industry setting Mastery of aseptic technique and experience in isolating primary T cells from PBMCs is essential Expertise in flow cytometry acquisition and analysis is a non-negotiable requirement Desired skills: Direct experience with non-viral transfection methods Comfortable using liquid-handling robotics to scale screening efforts Strong communication skills to present clear, data-driven updates Pay range: up to $50.81 per hour Only candidates available and ready to work directly as Aptask Global Workforce (AGW) employees will be considered for this position. If you have the described qualifications and are interested in this exciting opportunity, apply today! Aptask Global Workforce (AGW) ApTask Global Workforce (AGW) is a certified Minority and Veteran workforce solutions company. AGW delivers operational, clinical, lab and professional talent with a strong focus on healthcare and life sciences. The company supports clients with reliable staffing, program expertise and a commitment to quality, speed and consistent delivery. Benefits of working with ApTask Global Workforce include: Medical Dental Vision Sick Pay (for applicable states/municipalities) Our team stays close to the process and is here to guide you every step of the way. To learn more, please visit our website https://www.agwtalent.com/. ApTask Global Workforce is an Equal Opportunity Employer. Candidates will receive consideration without regard to their race, color, religion, sex, sexual orientation, gender identity, national origin, disability or status as a protected veteran.
